Composite

Part:BBa_K936024:Design

Designed by: Pui Yan Ho   Group: iGEM12_UC_Davis   (2012-10-02)


K206000+B0034+Reductase (aerobic)+B0034+Dehydrogenase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
    Illegal NheI site found at 789
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 65
    Illegal BamHI site found at 1567
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1039
    Illegal AgeI site found at 1240
    Illegal AgeI site found at 1839
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

SDM performed on glycolaldehyde reductase to produce the aerobic version of the enzyme.


Source

e. coli MG1655

References